[目的] 从海洋环境中筛选出能有效抑制细菌群体感应的活性菌株,为以致病菌群体感应为靶点的新型疗法提供新的天然产物资源.[方法] 以紫色杆菌(Chromobacterium violaceum)为报告菌,采用滤纸片法和双层软琼脂法相结合的筛选模型进行群体感应抑制活性菌的筛选.[结果] 通过对美国圣璜岛海域海绵中分离出来的272株海洋细菌群体感应抑制活性的筛选,得到了具有抑制紫色杆菌素产生的细菌51株,其中74号菌株抑制效果最好,具有进一步研究的价值.[结论] 海洋细菌中有很多具有抑制细菌群体感应效应的菌株,是天然群体感应抑制剂的潜在来源.%[Objective] Quorum sensing inhibitory activity of some marine bacterial isolates was investigated to provide potential novel origins of natural products for the promising antibacterial therapeutic approaches targeting on the quorum sensing system of some pathogens. [Methods] In this study, we used Chromobacterium violaceum as the report-strain to screen for the quorum sensing inhibitory activity from some marine bacterial isolates by disc diffusion assay and double-layer soft agar assay. [Results] In total, 272 bacteria strains isolated from the sponge tissues collected around the San Juan Island had been tested. The results showed that 51 isolates exhibited the quorum sensing inhibitory activity. Among the active strains, bacterium No.74 had the strongest activity, which is deserved the further study on the isolation and identification of quorum sensing inhibitors. [Conclusion] Many marine bacterial isolates exhibit quorum sensing inhibitory, which indicated that marine bacteria are also a potential source for the natural quorum sensing inhibitors.
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