等离子体射流灭活液体中铜绿假单胞菌的研究

摘要

[Objective] To determine the effect of atmospheric pressure plasma jet on the inactivation of Pseudomonas aeruginosa,and to explore the sterilization mechanism of atmospheric pressure plasma jet.[Methods] The bactericidal effect of atmospheric pressure plasma jet was determined by plate counting method.Treated cells were examined by fluorescence microscope and transmission electron microscope.Soluble proteins were quantified and analyzed by SDS-PAGE electrophoresis.[Results] More than 99.9％ P.aeruginosa cells were killed after the plasma treatment for 5 min.The cell wall and membrane systems of P.aeruginosa was broken as evidenced by leakage of cellular contents of proteins.Amounts of proteins was detected in supernatant after treatment for 2 min.[Conclusion] Atmospheric pressure plasma jet is able to destroy the cell integrity,which results in leakage of cellular contents and eventually kills P aeruginosa effectively.%[目的]测定等离子射流对铜绿假单胞菌(Pseudomonas aeruginosa)的灭活效果,探究低温等离子体射流的杀菌机理.[方法]采用平板计数法测定等离子体射流的杀菌效果,荧光显微镜、透射电镜观察等离子体作用后菌体结构的变化,蛋白浓度测定和SDS-PAGE电泳检测菌液上清液中可溶性蛋白的泄漏量.[结果]等离子体射流处理铜绿假单胞菌菌液5 min,杀灭率可达到99.9％以上.透射电镜观察可见细菌菌体结构发生改变,细胞壁、细胞膜损伤破裂,细胞内容物泄露.进一步对处理铜绿假单胞菌上清液中的蛋白质含量变化进行检测,结果显示随着处理时间的增加,上清液中蛋白质含量持续增加,在2 min时达到最大值.[结论]等离子体射流可以通过破坏细胞结构造成细胞质泄露,使其丧失正常的细胞功能,从而达到快速有效地杀灭铜绿假单胞菌的效果.