Studied the effect of pH, temperature and the additives on the bio-oxidation process, including the specific cell growth rate and the specific bio-oxidation rate of refractory gold concentrate by Acidithiobacillus ferrooxidans(At. ferrooxidans). Results showed that: the maximal specific growth rate was achieved at pH range of 1.1 ～ 2.0, and a temperature of 41 ℃. The maximal specific bio-oxidation rate was achieved at a pH range of 1. 1 ～ 1.4, and a temperature of 41 ～44℃. The peptone improved the concentrate bio-oxidation rate from 0. 41 ± 0. 02 g/( L · d) to 0. 92 ± 0.05 g/( L · d), and the cysteine 0. 41 ±0. 02 g/( L · d) to 0. 57 ±0. 03 g/( L · d). The optimal adding concentrates of them were 10 -3 g/mL and 6. 4 × 10 -5 mol/L, respectively. The best time for adding them was before the bacteria logarithmic phase.%以菌体的生长和生物氧化活性为指标,对酸性氧化亚铁硫杆菌氧化难浸金精矿过程中pH值和温度及有机添加剂的影响进行了研究.结果表明:当pH值为1.1～2.0,温度为41℃左右时菌体的比生长速率最大;当pH值为1.1～1.4,温度为41～44℃时,菌体比氧化速率最大.少量添加蛋白陈可使矿物氧化速率从0.41±0.02g/(L·d)提高为0.92±0.05 g/(L·d);少量半胱氨酸的加入可使矿物氧化速率从0.41±0.02 g/(L·d)提高为0.57±0.03 g/(L·d);两者的最佳添加浓度分别为10<'-3>g/mL和6.4×10<'-5>moL/L,最佳添加时间均为细菌生长进入指数生长期之前.
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