Effect of HK3 on immune invasion, proliferation and invasion of colon cancer cells

摘要

Objective:Investigate the effects of Hexokinase-3 on the proliferation,cell cycle,migration and immunologic invasion of RKO and HCT116.Methods:The expression levels of HK3 in tumor and normal colon samples were analyzed by bioinformatics.RKO and HCT116 were transfected with the control group(si-NC)and interference group HK3(si-HK3#1,si-HK3#2).CCK-8,Flow cytometry,wound healing and Transwell were used to examine the effects of interference with HK3 on the proliferation,cell cycle and migration of RKO and HCT116 cells.The expression levels of vimentin and E-cadherin,which are related to epithelial-mesenchymal transition(EMT),were detected by Western blot.Results:HK3 is associated with infiltration of multiple immune cells and co-expression with multiple immune checkpoints in colorectal cancer.Compared with the Control group(si-NC),the protein expression level of HK3 in interference group(si-HK3#1,si-HK3#2)was significantly decreased(P<0.05);the proliferation and migration of RKO and HCT116 cells were significantly inhibited(P<0.05);the cell cycle of RKO and HCT116 cells was arrested in S phase(P<0.05)and the expression of E-cadherin protein was increased,but the expression of vimentin protein was inhibited(P<0.05).Conclusion:HK3 affects the level of immunologic invasion of colon cancer.Interference with HK3 inhibits the proliferation,cell cycle and migration of colon cancer cells by inhibiting EMT behavior.