目的：探讨黄芩总黄酮苷元( SBTF)对肝脏的保护作用。方法将70只小鼠随机均分为7组(空白组、模型组、阴性对照组、联苯双酯阳性对照组及高、中、低剂量黄芩总苷元给药组)，每组10只，灌胃给药15天。高、中、低剂量给药组分别给黄芩总苷元400、200、100 mg/kg；阳性组给予联苯双酯200 mg/kg；阴性组给予2.0‰泊洛沙姆水溶液；模型组和空白组均给予生理盐水。第15天给药6 h后除空白组外均腹腔注射0.1‰四氯化碳油溶液。12 h后称重，取血，分离肝脏，拍照、制匀浆测定丙二醛( MDA)指标；全血离心后取血清测定肝功能指标丙氨酸氨基转移酶( ALT)和天门冬氨酸氨基转移酶( AST)。结果阳性组与高剂量给药组中小鼠肝脏外表光滑红润，无血块和斑点，但光泽差于空白组；中、低剂量给药组红润程度依次下降，并可见瘀斑渐次明显；阴性组和模型组外观苍白，可见成块淤血、瘀斑。模型组、阴性组AST、ALT、MDA指标与空白组相比明显升高；给药组AST、ALT、MDA指标按低、中、高剂量下降，与模型组相比差异有显著性。结论黄芩总黄酮苷元对肝脏有保护作用。%Objective To study the hepatic protection effect of Scutellaria Baicalensis Total Flavonoid (SBTF). Meth-ods Seventy male Kunming mice were randomly divided into seven groups:blank control group,model group,negative group, bifendate positive group and high-dose,middle-dose,low-dose group. Stomach perfusion was given for 15 days;High-dose,middle-dose,low-dose group were given SBTF 400mg/kg,200mg/kg,100mg/kg each once a day;positive group,negative group,model group and blank control group were given bifendate(200mg/kg),poloxamer solution(2. 0‰),normal saline separately. At the 15th day,6 hours after perfusion,all mice were intraperitoneally injected carbon tetrachloride oil resolution (0. 1‰) except the blank group,and 12h later,mice were weighed and taken for blood;their livers were separated,taking pictures,making homogenate to test MDA. The whole blood was centrifugalled and blood serum was to test ALT and AST and calculate liver index. Result For positive and high-dose group,the surface of mice’s liver was smooth and ruddy with no blood clot or spots,but a little dull com-pared with blank control group;middle-dose and low-dose group had less ruddy sequence with more obvious blood spots;negative and model group were pale,with block of clots and spots. AST,ALT,MDA of negative and model group were apparently higher than blank control group;decreases in the sequent low-dose,middle-dose,high-dose group,but not so evident as positive group.
展开▼
