SHP-1与c-kit基因在慢性髓系白血病中表达水平的研究

摘要

Objective To explore the expression of SHP-1 gene and c-kit gene in chronic myeloid leukemia and the function in leukemia conversion and prognosis. Methods Semi-quantity reverse transcriptase-polymerase chain reaction(RT-PCR) was used to examine the respective expression of SHP-1 mRNA and c-kit mRNA in the chronic and acute period of chronic myeloid leukemia and normal controls. Results The expression of SHP-1 mRNA in both the chronic period and acute period was less obvious than that of the normal controls, the difference had statistical signiifcance(P<0.05), but the difference between the chronic period and the acute period had no statistical signiifcance(P>0.05) . The expression of c-kit mRNA in the two periods was more obvious than that of the normal controls, the difference had statistical significance(P<0.05), and the expression in the acute period was more obvious than that in the chronic period, the difference had statistical signiifcance(P<0.05). Conclusion SHP-1 gene and c-kit gene may act as the indicators to judge chronic myeloid leukemia conversion and prognosis.%目的：探讨造血细胞磷酸酶（SHP-1）基因、c-kit基因在慢性髓系白血病患者中的表达及其与白血病转化和预后的关系。方法用半定量逆转录-聚合酶链反应法检测SHP-1 mRNA、c-kit mRNA分别在慢性髓系白血病慢性期、急变期及正常对照组中的表达。结果慢性期和急变期SHP-1 mRNA的表达均低于正常对照组，差异有统计学意义（P<0.05），慢性期和急变期比较差异无统计学意义（P>0.05）。慢性期和急变期c-kit mRNA的表达均高于正常对照组，差异有统计学意义（P<0.05），急变期c-kit mRNA的表达均高于慢性期，差异有统计学意义（P<0.05）。结论SHP-1基因与c-kit基因可作为判断慢性髓系白血病患者转归及预后的指标。