激素剂量及周龄对小鼠超数排卵的影响

摘要

目的：探讨不同剂量激素和不同周龄小鼠对超数排卵数量和囊胚率的影响，以期确定最适剂量和最佳周龄小鼠，获得更好的超数排卵效果。方法采用不同剂量孕马血清促性腺激素（ PMSG）和人绒毛膜促性腺激素（ hCG）对不同周龄小鼠进行超数排卵，统计胚胎个数及囊胚率。结果激素剂量为PMSG与hCG各7．5 IU组的超数排卵显著多于5．0 IU组（P＜0．05），但与10．0 IU 组差异无统计学意义，激素剂量为5．0 IU组囊胚率优于7．5 IU组和10．0 IU组，差异无统计学意义；不同周龄小鼠间超数排卵效果和囊胚率有一定差异，8周龄小鼠的超数排卵数量和囊胚率均高于其他各组。结论采用PMSG与hCG各7．5 IU对8周龄小鼠进行超数排卵处理效果最佳。%Objective To discuss the influence of different hormone doses and week-age for the superovulation and subsequent embryonic development in mice to make sure which were the optimal dose and the most appropri -ate week-age for superovulation .Methods The mice of different week-old were treated by different dosage of PMSG and hCG to induce superovulation , and then calculated the embryos number and blastocysts rate .Results Compared with other groups ,the amounts of embryos obtained in groups of different PMSG/hCG doses were sig-nificantly different and that of the 7.5 IU group was the highest (P<0.05).The amount of embryos obtained and the blastocyst rate in mice at age of 8 weeks was the highest and there were significant differences among all groups .Conclusion The age of 8 weeks and the dose of 7.5 IU PMSG/hCG are the best age and effective dose to induce superovulation in mice .