IL-10联合生长抑素对大鼠重症急性胰腺炎炎症相关细胞因子的抑制作用

摘要

目的：探讨白细胞介素10（IL-10）联合生长抑素对大鼠重症急性胰腺炎（SAP）炎性因子的调节作用。方法将27只SD大鼠依据随机数字表法分为三组：A组为空白对照组（n ＝6），B 组为SAP组（n＝6），C组为生长抑素联合IL-10肌肉注射治疗组（n＝15）。其中B组采用“逆行胆胰管注射法”制成SAP模型，C组在模型建立后即刻注射IL-10和生长抑素。三组均于术后处死大鼠且于第1、6、12小时留取血液标本，采用酶联免疫吸附法测定血清中炎性因子肿瘤坏死因子α（ TNF-α）、IL-6、IL-8的变化。结果 B组和C组第6小时、第12小时的 IL-6、IL-8、TNF-α水平均有升高，且呈上升趋势，差异有统计学意义（ P＜0．05），但 C 组较 B 组 IL-6、IL-8、TNF-α水平低，差异有统计学意义（P＜0．05）。结论 SAP时，应用IL-10联合生长抑素可降低血中 IL-6、IL-8、TNF-α的水平，是具有临床应用前景的治疗SAP的方法之一。%Objective To investigate the effects of interleukin 10 ( IL-10 ) combined with somatostatin treatment on theinflammatory factors in severe acute pancreatitis(SAP) rat model.Methods Twenty-seven SD rats were randomly divided into three groups:group A was control group(n=6),group B was SAP group (n=6),group C was somatostatin intramuscular injection in combination with IL-10 intramuscular injection treatment group(n=15).Group B was made into SAP models by retrograde cholangiopancreatography injec-tion method.Group C was immediately injected with IL-10 and somatostatin.Serum were collected from the groups 1 hour,6 hours,12 hours after treatment,ELISA assay of serum inflammatory factors ( TNF-α,L-6, IL-8) were performed.Results Increase of IL-6,IL-8,TNF-αlevels at 6 h,12 h were observed in both group B and group C,and a rising trend was observed,the difference was statistically significant(P <0.05),but IL-6, IL-8,TNF-αlevel of group C were lower than group B,the difference was statistically significant(P<0.05).Conclusion In SAP model,the application of IL-10 combined with somatostatin,could significantly inhibit serum levels of IL-6, IL-8, TNF-α, which is one of the treatments with perspectives for the clinical application.