肾移植受者红细胞 CD35、CD58、CD59表达变化

摘要

目的：观察肾移植受者移植前后外周血红细胞膜表面 CD35、CD58、CD59的表达情况。方法选择2013年1月—2015年1月肾移植受者25例及健康体检者25例,采用流式细胞术对肾移植术前、术后3、7、14 d 外周血红细胞膜表面免疫分子 CD35、CD58、CD59进行检测,观察红细胞免疫功能的变化情况。结果肾移植受者术前CD35、CD59表达明显低于对照组(P <0．05,P <0．01),CD35术后14 d 明显低于术前,CD58术后3 d 高于术前和术后14 d (P <0．05)。结论肾移植受者术前红细胞免疫功能低下,术后有所改善。红细胞免疫参与肾移植受者机体免疫功能的调节。%Objective To investigate the expressions of CD35, CD58 and CD59 on the surface of peripheral blood erythrocyte membrane of recipients before and after the kidney transplantation. Methods A total of 25 recipients with kidney transplantation during January 2013 and January 2015 were chosen as the observation group, and 25 healthy controls taking physical examinations were chosen as the control group. The flow cytometry was used to determine the ex-pressions of CD35, CD58 and CD59 on the surface of peripheral blood erythrocyte membrane before and on the 3rd , 7th and 14th d after the transplantation so as to observe the changes of immune function of red blood cells. Results The CD35 and CD59 expressions before the transplantation in the observation group were significantly lower than those in the control group (P < 0. 05, P < 0. 01). In the observation group, the CD35 expression on 14th d after the transplantation was significantly lower than that before the transplantation, and CD58 expression on 3rd d after the transplantation was sig-nificant higher than those before and on 14th d after the transplantation (P < 0. 05). Conclusion The immune function of red blood cells is weak in recipients before the transplantation, but the function can be recovered after the transplanta-tion. The erythrocytes immunity is involved in the regulation of immunologic function of recipients.