手工浓缩血小板三种分离方法的质量分析

摘要

Objective:To discuss the influence of different separation methods on concentrated platelet quality, in order to choose the best separation method for improvement of the platelet recovery rate and make sure clinical curative effect.Methods:90 bags whole blood, which Conform to the requirements of the concentrated platelet preparation, were selected as the research object randomly and was given the same centrifugal force and the same centrifugal time. Whole blood after centrifugation was separated by suspension method, plasma method and automatic blood component separator separation method separately. After the separation, concentrated platelet quality was analyzed.Results:The platelet separated by automatic blood component separator had the highest recovery rate and with the least number of red blood cells and white blood cells. Concentrated platelet recovery rate of plasma method was higher than the suspension method. After statistical analysis, statistical significance was found between groups of data (P < 0.05).Conclusions:Different methods of separation affect the recovery rate and quality of blood platelets.%目的：通过探讨不同分离方法对浓缩血小板质量的影响，选择最佳的分离方法提高血小板回收率，确保临床使用疗效。方法：随机抽取符合浓缩血小板制备要求的全血90袋，采用相同的离心力和离心时间，将离心后的全血分别采用悬挂法、分浆法及全自动血液成分分离机分离方法进行分离，对分离后的浓缩血小板进行质量分析。结果：采用全自动血液成分分离机分离的浓缩血小板回收率最高，且混入的红细胞及白细胞数量最少；而分浆法分离的浓缩血小板回收率高于悬挂法。经统计分析，各组数据间（P<0.05)有统计学意义。结论：不同的分离方法对血小板回收率及血液质量均有影响。