新型复合盖髓材料的体外细胞毒性研究

摘要

目的 检测新型复合盖髓材料磷酸钙-硅酸钙-泡铋矿复合水门汀(calcium phosphate-calcium silicate-bismuth compound cement,CASBi)的体外细胞毒性,并与临床常用盖髓材料的细胞毒性进行比较,为评价新型盖髓材料的生物相容性提供基础依据.方法 采用四甲基偶氮唑盐(MTT)比色法和琼脂覆盖法,评价CASBi和商品化盖髓材料Dycal、氢氧化钙糊剂(calcium hydroxide,CH)对人牙髓细胞的体外细胞毒性作用,结果 MTT和琼脂覆盖法均显示CASBi的细胞毒性为1级,Dycal和CH的细胞毒性为2级,统计结果显示,MTT实验中,CASBi与Dycal、CH的吸光度度值间存在显著性差异(P<0.05).结论 新型复合盖髓材料CASBi具有轻微的细胞毒性,对牙髓组织的影响还有待进一步研究.%Objective To evaluate the cytotoxic effect of a new pulp-capping agent. Methods Using agar overlay test and tetrazolium-based colorimetric assay (MTT) in vitro, the cytotoxicity of the three pulp-capping agents, a new compound agent - CASBi, calcium hydroxide cavity lining and pulp-capping material - Dycal, calcium hydroxide paste (CH) ,to human pulp cell were evaluated. Results Cytotoxicity of CASBi was 1 grade, while the cytotoxicity of Dycal and CH were 2 grade according MTT assay and agar overlay test. In the experimental groups the optical density (OD) of CASBi has significant difference compared with the other 2 materials (P;0. 05). Conclusion The new compound pulpcapping agent, CASBi had slight cytotoxity. Further study on the influence of the CASBi towards pulp tissue should be taken.