阿司匹林预处理对大鼠局灶性脑缺血/再灌注后IL-6、SOCS-3mRNA表达的影响

摘要

Objective After aspirin (ASA) pretreatment, the changes dyna-mically of the ischemic brain tissue IL-6 (IL-6) and cytokine signal transduction inhibitor-3 (SOCS-3mRNA) mRNA expression were studied after rat focal cerebral ischemia/reperfusion (C1/RP)" so as to clarify the role of aspirin, the possible mechanism of brain protection. Methods 95 male healthy SD rats were randomly divided into sham-operated group, ischemic control group, low-dose ASA group (l0mg. kg-1) and high-dose ASA group (150mg. kg-1). Modified suture method was used rat focal cerebral ischemia / reperfusion model. At 6h, 12h, 24 h, 72h, 7d time rats were tested neurological score. Taking ischemic cerebral hemisphere was measured in brain tissue IL-6 and SOCS-3mRNA content, and the surgical rats after 24h were used for TTC staining to infarct volume. Results After aspirin pretreatment, the neurological score of Small-dose and high-dose ASA group were significantly higher than that of the control group. 24h after infarct, the lesion volume decreased by 54. 48%, 30. 90% compared with the control group. The two experimental group's side of the brain lesions IL-6 levels decreased significantly compared with the control group. The two experimental groups side of the brain lesions SOCS-3mRNA levels significantly increased compared with the control group. Conclusion Aspirin pretreatment has effect to perfect the MCA-CI/RP neurological deficits. The possible mechanism and inhibition of inflammatory cytokines IL-6 production and increases SOCS-mRNA related, low dose of ASA is superior to high-dose ASA group.%目的 探讨阿司匹林(Aspirin,ASA)预处理后,对大鼠局灶性脑缺血/再灌注(cerebral ischemia/reperfusion,CI/RP)缺血脑组织保护作用的可能机制.方法 将雄性健康SD大鼠95只随机分为假手术组、缺血对照组、小剂量ASA组(10mg/kg)、大剂量ASA组(150mg/kg).采用改良线栓法制作大鼠CI/RP.术后分别在6、12、24、72h和7d时间点进行神经功能评分,白细胞介素6(IL-6)、细胞因子信号转导抑制因子(SOCS-3mRNA)含量检测,用术后24h的大鼠进行TTC染色计算脑梗死体积.结果 阿司匹林预处理后,小、大剂量ASA组大鼠神经功能评分较对照组明显升高(P＜0.05),24h梗死病灶体积与对照组比较分别减少54.48％和30.90％,病灶侧脑组织IL-6含量较对照组明显下降(P＜0.05),SOCS-3mRNA含量较对照组明显上升(P＜0.05),在术后12h～3d与对照组相比较均有显著性差异(P＜0.05).结论 ASA药物预处理对脑缺血/再灌注损伤有保护作用,其可能机制与抑制IL-6的产生和上调SOCS-mRNA 表达有关,小剂量ASA作用优于大剂量的ASA.