幽门螺杆菌感染及核转录因子-κBp65表达与胃癌的关系

摘要

Objective To investigate the relationship between helicobactor pylori infection and NF-?Bp65 expression in gastric cancer and precancerous lesions,and to study the possible mechanism of H. Pylori in gastric cancinogenesis. Methods 30 cases of chronic superficial gastritis specimens.30 cases of chronic atrophic gastritis specimens, 30 cases of intestina metaplasia specimens, 40 cases of dysplasia specimens and 80 cases of gastric carcinoma specimens were obtained Samples were fixed and embedded in paraffin as usual. Slides were used for HE and sp immunohistochemical staining or Methylene Blue staining,the latter was used to detect the expression of NF-?Bp65 and H. Pylori infection. NF-icBp65 expression is highly related with H. Pylori infection NF-icBp65expression is highly lrelated with H. Pylori infection. Results In CSG and GC,there was no significant difference in the positive rate of NF-icBp65 between H. Pylori positive patients and H. Pylori negative patients (P>0. 05). In CAG,IM and Dys .their expression level were intensive in H. Pylori positive patients when compared with H. Pylori negative patients(42. 1% vs 7. 7%,67. 9% vs 18. 2%,78. 6% vs 25. 0%). Conclusion The expression of NF-icBp65 is upregulated in the process of gastric cancer, NF-icBp65 possibly is helpful to the gastric cancer early diagnosis.%目的 探讨胃黏膜幽门螺杆菌(Helicobactor pylori,Hpylori)感染、核转录因子-κBp65(NF-κBp65)表达与胃癌发生、发展的关系.方法 胃癌(GC)标本80例,非典型增生(Dys)40例,萎缩性胃炎(CAG)30例,肠上皮化生(IM)30例,浅表性胃炎(CSG)30例,应用免疫组化方法测定NF-κBp65蛋白的表达,用亚甲兰染色检测Hpylori.结果 CSG、GC中Hpylori阳性和阴性病例NF-κBp65表达差异无显着性(P＞0.05),但在CAG、IM、Dys的Hpylori阳性病例中NF-κBp65表达显著高于Hpylori阴性病例(42.1％ vs 7.7％、67.9％ vs 18.2％、78.6％ vs 25.0％).结论 Hpylori感染诱导NF-κBp65表达发生在胃癌形成的早期阶段.