老年痴呆模型中人参皂苷Rg1预处理对大鼠脑片中突触素表达的影响

摘要

Objective To investigate the effects of ginsenoside Rg1 on the expressions of SYN in Alzheimer's disease of rat brain slices induced by okadaic acid (OA),and explore the mechanisms of gensenoside Rg1 in Alzheimer's disease.Methods 400 μm thick brain slices which were cut from brains of 5-week-old SD rats,were randomly divided into control group,sham group,low-dose ginsenoside Rg1 group,medium-dose ginsenoside Rg1 group and high-dose ginsenoside Rg1 group,with 10 slices in each group.All these brain slices were cultured with artificial cerebrospinal fluid (ACSF).And brain slices in the ginsenoside R1 groups were administrated with the ginsenoside Rg1 (60,120 and 240 μmol/L respectively) in ACSF.The concentration of OA in each group was 1 μmol/L.Expressions of SYN proteins in brain slices were determined by immunohistochemical method,and the results were analyzed by image acquisition and analysis system.Results The expression of SYN protein was decreased (P＜0.05) in the sham group,compared with the control group.The expression of SYN protein was increased (P＜0.05) in the ginsenoside Rg1 groups,compared with the sham group.Conclusion Ginsenoside Rg1 can increasing the expression of SYN in Alzheimer's disease to protect the brain and slow down the aging process.%目的 通过冈田酸(OA)诱导大鼠脑片老年痴呆模型,观察用人参皂苷Rg1干预后对大鼠脑片中突触素(SYN)表达的影响,以探究中药人参皂苷Rg1抗老年痴呆的作用机制.方法 将冈田酸作用于SD大鼠脑片,构建老年痴呆病理模型.随机分为空白组、模型组和人参皂苷Rg1低、中、高药物干预组,每组10张脑片.脑片置人工脑脊液中培养,干预结束后固定切片处理.采用免疫组织化学染色、图像分析技术等方法,检测皮层和海马SYN的表达水平.结果 与空白组比较,模型组皮层和海马内SYN的蛋白表达量明显减少(P＜0.05),人参皂苷Rg1各浓度组SYN的蛋白表达量增加(P＜0.05),并呈浓度依赖性.结论 人参皂苷Rg1可能通过影响突触素的活性表达,发挥抗老年痴呆神经退行性变的作用,减缓大脑的老化过程,从而起到保护大脑的作用.