宫颈癌整合素αVβ5的表达及其与血管生成和侵袭性转移的关系

摘要

Objective The present study aims to investigate the mechanism of integrin aV?5 in the angiogenesis and invasive metastasis of cervical carcinoma. Methods Seventy-five cervical carcinoma specimens excised from patients in Yantan Yu Huang Ding Hospital from June 2008 to June 2010 were studied. They were divided into two groups, namely, the carcinoma in situs of cervix (n= 15) and invasive carcinoma ( n = 60) groups. In addition, 15 normal cervical specimens from patients with panhysterectomy due to hysteromyoma during the aforementioned period were randomly selected as the control group. The immunohistochemical PV two-step method was used to examine the integrin ocV?5 expression levels and microvessel densities (MVDs) in 15 cases of normal cervical tissues, 15 cases of carcinoma in situs of cervix, and 60 cases of invasive cervical carcinoma (ICC) tissues. Results The positive integrin aV?5 expression levels (P<0.05) and MVDs (P<0.01), indicated by CD105, in the ICC group were significantly higher than those in the carcinoma in situs of cervix and control groups. Moreover, the integrin aV?5 expression level and MVD in stage II cervical cancer were significantly higher than those in stage I (P<0. 05). In histological grade cervical cancer, the positive integrin 0.05). Conclusion The enhanced integrin aV?5 expression level and MVD may play an important role in the generation and development of cervical carcinoma. Moreover, integrin aV?5 was found to be closely related to angiogenesis and invasive metastasis.%目的 探讨整合素αVβ5在宫颈癌血管生成和侵袭性转移中的作用机制.方法 选择2008年6月-2010年6月在烟台毓璜顶医院手术切除的宫颈癌患者75例,分为宫颈原位癌组(n=15)和浸润癌组(n=60),另随机选择15例同期因子宫肌瘤行全子宫切除的正常官颈标本作为对照组.采用免疫组织化学PV两步法(非生物素)分别测定15例正常宫颈、15例宫颈原位癌、60例宫颈浸润癌组织中整合素αVβ5的表达及微血管密度(MVD).结果 官颈浸润癌整合素αVβ5的阳性表达明显高于原位癌组和对照组(P＜0.05).CD105标记的官颈浸润癌MVD值较原位癌组和对照组明显增加(P＜0.01).官颈癌Ⅱ期整合素αVβ5的阳性表达较Ⅰ期明显增强,MVD较Ⅰ期明显增高,差异有统计学意义(P＜0.05).组织学分级G2,Q整合素αVβ5的阳性表达明显高于G1,且MVD较G1明显增高,差异有统计学意义(P＜0.05).有官颈癌淋巴结转移者整合素αVβ5的阳性表达明显高于无淋巴结转移者(P＜0.05),而二者MVD比较差异无统计学意义(P＞0.05).官颈鳞癌整合素αVβ5和MVD的表达与腺癌相比差异无统计学意义(P＞0.05).结论 整合素αVβ5表达增强和MVD增高可能在官颈癌发生、发展中发挥了重要作用.整合素αVβ玛与肿瘤血管生成和侵袭性转移密切相关.