气体信号分子硫化氢对高脂饲养大鼠肝脏脂代谢的调节作用

摘要

Objective To investigate the effects of hydrogen sulfide on lipid metabolism in liver of high-fat-fed (H-FD) rats. Methods Twenty-four male SD rats were randomly divided into control group, H-FD group and H-FD+NaHS group (8 each). Rats were sacrificed after feeding for eight weeks, and hepatic tissue was obtained and frozen sectioned. They were stained with oil red O, Philipin and HE. The oil red O and Philipin-dyed pictures were measured by image analysis system. Results Oil red O staining showed that the cell shape and structure of hepatic tissue were normal in control group, and massive diffuse lipid deposition and damaged structure in hepatic lobule were found in H-FD group. The lipid deposition decreased significantly, and the damage of hepatic lobule also ameliorated, in H-FD+NaHS group (5818. 79+297. 45) compared with H-FD group (62612. 70±756. 46, P<0. 01). Philipin staining showed that the fluorescence signal of total cholesterol was negative in control group, and it was positive in H-FD group where the signal was strong and well distributed. The fluorescence intensity of total cholesterol was significantly weakened in H-FD+NaHS group (52. 13 ±3. 70) compared with H-FD group (201. 21+3.18, P<0.01). Macroscopic examination with HE staining showed that the cell shape and structure of hepatic tissue were normal in control group; the hepatocytes contained big lipid droplets, becoming swollen and rounded with obviously injured central area of hepatic lobule in H-FD group; while the lipid droplets in hepatocytes was distinctly decreased in H-FD+NaHS group, and the shape and structure of hepatocytes recovered to certain extent. Conclusion Exogenous hydrogen sulfide might show a novel regulatory effect on abnormality of lipid metabolism in liver of high-fat-fed rats.%目的 探讨气体信号分子硫化氢(H2S)对高脂饲养大鼠肝脏脂代谢的调节作用.方法 雄性SD大鼠24只,随机分为对照组、高脂组和高脂+NaHS组,每组8只.喂养8周后处死大鼠,留取肝组织冰冻切片,分别进行油红O染色、总胆固醇PHILIP-IN染色及HE染色,对油红O染色和总胆固醇染色结果采用积分光密度计数进行图像分析.结果 经油红O染色后在光镜下观察,对照组肝组织的细胞形态和结构正常;高脂组肝细胞和细胞间隙有脂质大量弥漫沉积,肝小叶结构破坏;高脂+NaHS组脂质沉积(5818.79±297.45)较高脂组(62 612.70±756.46)明显减少(P＜0.01),肝小叶结构破坏程度减轻.对照组总胆固醇PHILIPIN荧光染色无阳性信号显示;高脂组总胆固醇PHILIPIN荧光染色(201.21士3.18)呈明蓝色荧光阳性信号,信号强烈且分布较均匀;高脂+NaHS组明蓝色荧光阳性信号强度(52.13±3.70)明显减弱,与高脂组比较差异有统计学意义(P＜0.01).HE染色光镜下观察见对照组肝细胞形态和结构正常;高脂组大量肝细胞含有脂肪滴,将胞核推挤到细胞一侧,肝细胞肿大变圆,小叶中央区受累明显;高脂+NaHS组肝细胞内脂肪滴明显减少,肝细胞形态和结构有所恢复.结论 外源性H2S对大鼠高脂饮食所致肝脏脂质代谢异常有明显改善作用.