方格星虫多糖对低剂量电离辐射、一氧化碳、苯和噪声复合损伤大鼠的保护作用观察

摘要

目的 观察方格星虫多糖对低剂量电离辐射、一氧化碳、苯和噪声等有害环境因素复合损伤大鼠的保护作用.方法 健康成年SD大鼠50只,随机分为正常对照组、模型对照组及方格星虫多糖低、中、高剂量组.给药组大鼠每天于γ射线照射前经口给予方格星虫多糖70、140、280mg/(kg.d),连续7d.模型对照组给予等体积0.9％ NaC1.试验结束后处死动物,全自动血细胞计数仪检测外周血细胞计数,紫外分光光度法测定骨髓DNA含量,试剂盒检测血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并计算主要脏器(肝脏、脾脏、胸腺)指数.结果 与模型对照组比较,方格星虫多糖处理后外周血红细胞、血小板计数及血细胞比容、血红蛋白水平、SOD活性均显著升高(P＜0.05,P＜0.01),白细胞有升高趋势,MDA含量明显下降(P＜0.05),骨髓DNA含量显著升高(P＜0.05),而肝脏指数、脾脏指数、胸腺指数均无明显变化.结论 方格星虫多糖对有害环境因素复合损伤大鼠具有升高白细胞、血小板,提高抗氧化能力,促进骨髓损伤修复等保护作用.%Objective To investigate the protective effects of Sipunculus nudus polysaccharides (SNPS) on rats injured by low-dose irradiation combined with carbon monoxide, benzene and noise. Methods Fifty SD rats were randomly divided into normal control group, model control group, 70mg/(kg·d) SNPS group (SNPS 70 group), 140mg/(kg·d) SNPS group (SNPS 140 group) and 280mg/(kg·d) SNPS group (SNPS 280 group). SNPS was given intragastrically once a day before y irradiation for 7 days. Rats in model control group were given the same volume of 0.9% NaCl for 7 days. All the rats were sacrificed 7 days later. Peripheral blood cell count of all the rats was done with blood cytometry. DNA in bone marrow cells was determined by ultraviolet spectrophotometry. The activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) in serum were determined by the reagent kits. The parameters of function of main organs (liver, spleen and thymus) were also determined. Results Compared with model control group, peripheral blood PLT, RBC, HCT and HGB increased significantly (P<0.05 or P<0.0l), WBC increased slightly, and SOD activity and DNA in bone marrow increased significantly in SNPS groups, while the content of MDA decreased in SNPS groups compared with that in model control group (P<0.05). No significant change was found in the main organs (liver, spleen and thymus) indexes. Conclusion SNPS may give a protective effect on rats injured by combined injurious factors by increasing WBC and PLT in serum, improving antioxidant activity and promoting the repair of injured bone marrow.