Objective:To investigate relationships among age, gender and changes of the pupil diameter and response rate in different lights. Methods:410 cases (820 eyes) and their families were selected as the research subject, and divided into low age group (6-40 years old), the middle age group (41-60 years old,), and high age group (over 61 years old). The low age group com-prised 145 people(290 eyes), including male of 85 people (170 eyes) and female of 70 people (140 eyes), the average age 22. 56± 11. 23 years old;the middle age group included 150 people(300 eyes), including male of 80 people (160 eyes) and female of 70 peo-ple (140 eyes), the average age 44. 73±5. 36 years old;and the high age group had 115 people(230 eyes), including male of 56 peo-ple (112 eyes) and female of 54 people(108 eyes), the average age 64. 36±4. 56 years old. Results: Under the same light, there were no statistical differences in thepupil diameter and response rate among the female and the male (P>0. 05);however, there were statistical differences between each two groups s (P<0. 05). Moreover, with the enhancement of light, the differences of the pupil di-ameter and response rate were statistically significant (P<0. 05). Conclusions: Under different lights, the pupil diameter and re-sponse rate change with age and sex. Therefore, it has a guiding significance for the close observation of pupil diameter and response rate changes by the clinicians to observe the patients' conditions and prognosis.%目的：：探讨不同光线下研究对象的年龄、性别与瞳孔直径及反应速率变化的关系。方法：选取410例(820眼)患者及其家属作为研究对象。以年龄不同将以上研究对象分为低年龄组(6~40岁)、中年龄组(41~60岁)和高年龄组(61岁以上)。低年龄组研究对象145例(290眼),男性85例(170眼),女性70例(140眼),平均年龄(22.56±11.23)岁；中年龄组研究对象150例(300眼),男性80例(160眼),女性70例(140眼),平均年龄(44.73±5.36)岁；高年龄组研究对象115例(230眼),男性54例(108眼),女性56例(112眼),平均年龄(64.36±4.56)岁。结果：同一光线下,研究对象的男、女性别之间瞳孔直径,反应速率差别无统计学意义(P>0.05)；同一光线下,3组研究对象两两比较,发现随着年龄增高,瞳孔直径,反应速率具有统计学意义(P<0.05)；随着光线的增强,瞳孔直径,反应速率具有统计学意义(P<0.05)。结论：不同光线下瞳孔直径及反应速率变化与研究对象的年龄,性别相关,密切观察瞳孔及反应速率变化对临床医生观察就诊者病情及转归具有指导性意义。
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