赖氨酸对甲基苯丙胺神经毒性的保护作用

摘要

Objective To investigate the protective effect of lysine against methamphetamine - induced neurotoxicity in C6 cells. Methods C6 cells were randomly divided into control group, methamphetamine(2 mM, 3 mM, 4mM) group, lysine group, methamphetamine + lysine group. To determine the oxidative damage induced by methamphetamine, catalase ( CAT) and glutathione peroxidase (GPx) were detected in C6 cells. To estimate apoptosis, flow cytometry analysis using annexin V - FITC and propidium iodide staining as well as detection for caspase - 3 and Bel - 2 proteins were performed after methamphetamine exposure for 48 hours. Results Lysine reversed the change of the form of C6 cells caused by methamphetamine and greatly improved CAT and GPx activities. It also reversed the expression of Bel - 2 apoptosis protein induced by methamphetamine. Conclusions Lysine can ameliorate methamphetamine - induced oxidative insult and apoptosis in C6 cells, probably due to its antioxidant activity rather than the activation of GAB A receptors.%目的 评价赖氨酸对甲基苯丙胺所致神经细胞氧化损伤和凋亡的保护作用.方法 选取大鼠神经胶质细胞的C6细胞株体外培养后,随机分为对照组、甲基苯丙胺(2 mM、3 mM、4 mM)组、赖氨酸组、甲基苯丙胺+赖氨酸组.通过研究C6细胞的过氧化氢酶(CAT)活性,谷胱甘肽过氧化物酶(GPx)活性,凋亡和凋亡相关蛋白(caspase-3、Bcl-2)的表达,以评价赖氨酸的作用.结果 赖氨酸可以部分逆转甲基苯丙胺所致的C6细胞形态的改变,显著提高CAT和GPx的活性,可以逆转甲基苯丙胺诱导的凋亡蛋白Bcl-2的表达.结论 赖氨酸对甲基苯丙胺所致的C6细胞的神经毒性具有保护作用,其机制是抑制氧化应激而不是通过活化GABA受体起作用的.