CCK-8法与MTT法检测兔成纤维细胞活性的比较研究

摘要

目的：探讨 CCK-8法和 MTT 法检测兔成纤维细胞活性的最佳实验条件,并比较其优略.方法：以传至第3代的兔成纤维细胞为研究对象,分别用 CCK-8法和 MTT 法检测兔成纤维细胞的增殖活性.结果：MTT 法的最佳检测波长490 nm,CCK-8法最佳检测波长为450 nm,两法的最佳检测时间均为加入试剂后4 h.适宜检测的细胞数量范围为2×103/ml ~1×105/ml 个.结论：CCK-8法较 MTT 法检测的灵敏度高,准确性好,是一种优于 MTT 法的检测兔成纤维细胞增殖活性的方法.%Objective:To investigate optive experiment conditions between MTT and CCK-8 in Rabbit fibroblasts,and to compare which was better.Method:Rabbit fibroblasts of PC3 cell were studied.The activity of rabbit fibroblast was detected respectively with CCK-8 and MTT.Result:For MTT assay,the best wavelength was 490 nm;and the best wavelength of CCK-8 assay was 450 nm.The best test both were 4 h after adding reagent,the number of detect Rabbit fibroblasts of PC3 cell was within the range of 2×103/ml-1×105/ml.Conclusion:CCK-8 assay is better than MTT assay in the aspects of detection sensitivity and accuracy,and CCK-8 is superior to MTT in Rabbit fibroblasts proliferation assay.