PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值

摘要

Objective:To investigate the value of PCR reverse hybridization membrane application in screening of CT,UU,NG three kinds of STD. Method:1486 suspected patients with genitourinary tract infections were detected by PCR reverse hybridization membrane and fluorescence quantitative PCR(FQ-PCR),and test results were compared with culture results. Result:FQ-PCR detection of CT,UU,NG was higher than culture(P<0.01). PCR reverse hybridization membrane,culture and FQ-PCR positive rate difference was not statistically significant. PCR reverse hybridization membrane,FQ-PCR detection sensitivity difference was not statistically significant,but the film PCR reverse hybridization assay specificity was higher than FQ-PCR(P<0.01). Conclusion:PCR reverse hybridization in screening of CT,UU,NG,is accurate,rapid,and specific is higher than FQ-PCR,worthy of promotion apply.%目的：探讨PCR反向膜杂交法在CT、UU、NG三种性病筛查中的应用价值。方法：对1486例疑为泌尿生殖道感染患者分别采用PCR反向膜杂交法及荧光定量PCR（FQ-PCR）检测，并将检测结果与培养结果比较。结果：FQ-PCR检测CT、UU、NG阳性率高于培养法，比较差异有统计学意义（P<0.01）；PCR反向膜杂交法与培养法及FQ-PCR检测阳性率比较差异均无统计学意义；PCR反向膜杂交法、FQ-PCR检测敏感性比较差异无统计学意义，但PCR反向膜杂交法检测特异性高于FQ-PCR，比较差异有统计学意义（P<0.01）。结论：PCR反向膜杂交法筛查CT、UU、NG准确，快速，且筛查特异性高于FQ-PCR，值得临床推广适用。