首页> 外国专利> - Y A DIAGNOSTIC METHOD FOR SNP IN Y CHROMOSOME USING PCR-REVERSE BLOT HYBRIDIZATION ASSAY AND USE OF THE SAME

- Y A DIAGNOSTIC METHOD FOR SNP IN Y CHROMOSOME USING PCR-REVERSE BLOT HYBRIDIZATION ASSAY AND USE OF THE SAME

机译:-Y-PCR反向杂交杂交测定Y染色体中SNP的诊断方法及其使用

摘要

The present invention relates to a method for providing information for diagnosing single nucleotide polymorphism (SNP) present on the Y chromosome, including a) a step of separating DNA from a sample; b) a step of amplifying, by using PCR, a Y chromosome haplogroup marker gene fragment using each primer pair from the DNA; and c) a PCR-reverse blot hybridization step of hybridizing a solid support to which an oligomeric probe for detecting the Y chromosome haplogroup marker gene is attached and the PCR amplification product obtained in step b). The present invention also relates to a composition and/or kit thereof for use in the method.
机译:本发明涉及一种用于提供信息以诊断存在于Y染色体上的单核苷酸多态性(SNP)的信息的方法,包括:a)从样品中分离DNA的步骤; b)通过使用PCR,使用来自DNA的每个引物对,扩增Y染色体单倍体标记基因片段的步骤; c)将固定有用于检测Y染色体单倍体标记基因的寡聚探针的固相支持体与在步骤b)中得到的PCR扩增产物杂交的PCR-反向印迹杂交步骤。本发明还涉及用于该方法的组合物和/或其试剂盒。

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