TCT联合HPV E6/E7 mRNA定量检测宫颈上皮内瘤变的临床应用价值

摘要

目的：探讨利用HPV E6/E7 mRNA检测技术检测妇科子宫颈上皮内瘤变的临床应用价值。方法：对69例TCT检查结果为宫颈上皮内瘤变的患者，应用HPV E6/E7 mRNA检测技术对感染HPV病毒中的癌基因片段E6、E7转录子做出定量检测。结果：>ASC-H组E6/E7 mRNA转录阳性率、转录数分别为60.6%、（18285.42±1487.71），均高于≤ASC-H组的33.3%、（2668.43±681.39），差异均有统计学意义（P<0.05）；宫颈细胞学检查为宫颈癌的HPV E6/E7 mRNA转录数明显高于其他类型，差异有统计学意义（P<0.05）。结论：宫颈上皮内瘤变与E6/E7 mRNA转录具有相关性，即转录数与病变的进展程度及细胞的恶性转化密切相关，在宫颈癌筛查中检测HPV E6/E7 mRNA的转录具有潜在价值。%Objective:To discuss the clinical application value of quantitative detection the HPV E6/E7 mRNA in cervical intraepithelial neoplasia.Method:In 69 cases of TCT examination results for cervical intraepithelial neoplasia,use of HPV E6/E7 mRNA detection technology for the infection of HPV virus in the cancer gene fragment E6,E7 transcription to make quantitative detection.Result:>ASC-H group HPV E6/E7 mRNA transcription positive rate(60.6%)was higher than the≤ASC-H group(33.3%),>ASC-H group HPV E6/E7 mRNA transcription number (18 285.42±1487.71)was higher than the≤ASC-H group(2668.43±681.39),the differences were statistically significant(P<0.05).Cervical cytological examination for cervical cancer HPV E6/E7 mRNA transcription number was significantly higher than other types,the difference was statistically significant(P<0.05).Conclusion:The cervical intraepithelial neoplasia is closely related to the E6/E7 mRNA transcription,that is,and that means the HPV E6/E7 mRNA transcription affect the the progression of lesions and malignant transformation of cells, quantitative detection the HPV E6/E7 mRNA has potential clinical application value in the screening of cervical intraepithelial neoplasia.