基质金属蛋白酶2，9，12与慢性阻塞性肺疾病的临床研究

摘要

目的：探讨慢性阻塞性肺疾病（COPD）与血清基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）、基质金属蛋白酶12（MMP-12）的相关性。方法：选取COPD患者80例，其中急性加重期（AECOPD）40例，临床缓解期40例，健康对照者45例，采用双抗夹心ELISA法测定所有研究对象的血清MMP-2、MMP-9、MMP-12含量，并做统计学分析。结果：COPD患者血清MMP-2、MMP-9、MMP-12水平均明显高于健康对照者，差异均有统计学意义（P<0.05或P<0.01）；且AECOPD患者血清MMP-2、MMP-9、MMP-12水平显著高于临床缓解期患者（P<0.01）。稳定期COPD患者血清MMP-2、MMP-9、MMP-12水平与第1秒用力呼气容积（FEV1）成负相关（r=-0.529，P<0.01；r=-0.385，P<0.05；r=-0.627，P<0.01）。结论：COPD患者血清MMP-2、MMP9、MMP-12水平均明显升高，且与疾病严重程度成正相关。%Objective:To investigate the correlation of patients with chronic obstructive pulmonary disease(COPD) with matrix metalloproteinase2(MMP-2),matrix metalloproteinase9(MMP-9) and matrix metalloproteinase 12(MMP-12) in the serum.Method:Double-antibody sandwich method (ELISA) was used to measure the concentrations of MMP-2,MMP-9,MMP-12 in the serum of 80 patients with COPD,including acute exacerbation (AECOPD) for 40 cases and clinical remission for 40 cases.45 healthy people were selected as the healthy control group.The statistical analysis was conducted.Result:The concentrations of MMP-2,MMP-9, MMP-12 in patients with COPD were significantly higher than those of the healthy control group,the differences were statistically significant(P<0.05 orP<0.01).These index in AECOPD group were significantly higher than those in the clinical remission group(P<0.01).The concentrations of MMP-2,MMP-9,MMP-12 in stable COPD patients were passively correlated with forced expiratory volume in first second(FEV1)(r=-0.529, P<0.01;r=-0.385,P<0.05;r=-0.627,P<0.01).Conclusion:The serum level of MMP-2,MMP-9,MMP-12 in COPD patients are obviously higher,and they are positively correlated with the severity of the disease.