固定化青霉素酰化酶合成阿莫西林活性测定

摘要

The level of activity of immobilized penicillin acylase is the important factor which affects the speed of enzymatic synthesis of amoxicillin, thus it plays signiifcant role how to determine synthetic activity of immobilized penicillin acylase. Currently, the determination of activity of penicillin acylase is focus on hydrolytic activity of penicillin acylase. But there is lack of the reports on determination in activity of synthesis of amoxicillin, which leads to the fact that fast determination of the synthesis speed from penicillin acylase to amoxicillin may be impossible and the production cannot be guided from theory. In this article, the activity of amoxicillin synthesized from penicillin acylase was deifned and the method for measuring the activity was studied. It was shown that this method is easy to operate and the result is accurate and reliable. Moreover, by using this method the activities of penicillin acylases produced from different factories are easy to be compared so that the comparing results can be used as guidance for production.%固定化青霉素酰化酶的活性高低是影响酶催化合成阿莫西林速度的决定性因素，如何测定固定化青霉素酰化酶的合成活性起着至关重要的作用。目前，对青霉素酰化酶活性的测定主要集中在青霉素酰化酶的水解活性，而对其合成阿莫西林活性的研究较少，这导致不能快速的鉴别青霉素酰化酶合成阿莫西林的快慢，不能有效的指导生产。文章对固定化青霉素酰化酶合成阿莫西林的活性进行了定义，并研究了合成活性的测定方法，该测定方法准确可靠，操作简捷，便于比较不同厂家生产的青霉素酰化酶活性的高低，对工业生产起指导作用。