目的：运用纤维蛋白胶（FG）结合神经生长因子（NGF）包裹 SD 大鼠坐骨神经断端，通过纤维蛋白胶控释 NGF 以促进神经纤维再生并减少神经吻合口瘢痕形成。方法：SD 大鼠24只，随机分为2组，每组12只。A 组：大鼠坐骨神经切断后神经外膜直接缝合，然后吻合口包裹 FG/NGF 凝胶，B 组：神经外膜直接缝合，吻合口未包裹凝胶。分别于术后第1、3、5、7周行术侧足迹实验检测，术后2月行术侧三头肌湿重恢复率测量、电生理测定、甲苯胺蓝染色、透射电镜分析等。结果：同一时间段两组间比较，各指标 A 组明显好于 B 组。结论：NGF 缓释凝胶可以促进大鼠坐骨神经离断损伤的修复并防止吻合口与周围组织粘连。%Objective:To evaluate the ef ect of the control ed release of biodegradable FG ,nerve growth factor (FG/NGF)on peripheral nerve defect.Methods :towenty -four Sprague2 Dawly rats were randomly divided into two groups,FG/NGF(group A ,n =12),Stitching of combine (group B ,n =12).Each rat underwent right sciatic nerve cuting into two pieces,then these defects were repaired in the two dif erent experimental groups.The process of the peripheral nerve regeneration was evaluated at 2 months fol owing operation,and the evaluated items were footprinting test,triceps weight ,electrophysiological ,Immunohistochemistr ,Methylene,Blue trihydrate axon morphologic assessment of the outcome of nerve regeneration.Results:Nerve regen-eration in group A was comparable with that seen in natuai Nerve and was significantly superior to that in B groups in al indexes.Conclusion::FG/NGF control ed release was a potential ideal, that can ef ectively promote nerve regeneration after sciatic nerve defect of rats.The ef ect was as good as that of natual Nerve.
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