We investigated the effects of temperature, luminosity, photoperiod, and diet on spawning, hatching, and larvae rearing of anemonefish Premnas biaculeatus. When the temperature was 26 ℃, with a photoperiod of 14L：10D, the luminosity of 500 lx and 2 000 lx had no different effects on spawning interval and fertilization, but showed obvious effects on the development of fertilized eggs. The time of hatching was longer and the hatching rate was lower with lower larvae survival under 500 lx. Twenty-six 26℃ was showed to be a suitable temperature to spawning, hatching, and larvae rearing. But the hatching rate and larvae survival decreased and the spawning interval delayed when the temperature was 24℃. PUFA-enriched broodstock diet was required for high hatching rate and husky larvae, and PUFA-enriched Brachionus plicatilis was necessary for larvae growth.%为了解棘颊雀鲷(Premnas biaculeatus)在人工饲养条件下繁殖和仔鱼培养对光照、水温及饵料的要求,采用实验生物学的方法,在严格控制的条件下培养亲鱼、胚胎和仔鱼.结果表明,在(26±1)℃和14L:10D的光暗周期下,2 000 1x和500 1x两种光照强度对亲鱼产卵周期和卵的受精率并没有明显的影响,但是对受精卵的发育有明显的影响,表现在低光照条件下受精卵的孵化时间延长、受精卵孵化率低和初孵仔鱼的存活率极低.温度的变化则对亲鱼产卵、胚胎发育以及仔鱼的存活率都有明显的影响,26℃是亲鱼产卵、受精卵孵化和仔鱼生长的适宜水温.当温度下降到24℃左右时,亲鱼的产卵间隔时间明显延长、孵化时间延长、孵化率降低、孵化仔鱼成活率低.28℃也会造成仔鱼大量死亡.亲鱼和仔鱼饵料中不饱和脂肪酸(PUFA)的含量对受精卵孵化率、初孵仔鱼体长和仔鱼成活率都有明显的影响.添加组的各项结果都明显优于未添加组.
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