In this paper, the activation characteristics, cryopreservation program optimization, short-term preserva-tion and fertilization of red spotted grouper sperm were studied in detail. The results showed that seawater with specific gravity of 1.018 and temperature of 25℃ has the best activating effect and the sperm motility was (82.50±4.18)%. Frozen samples with 7 cm height above the liquid nitrogen surface reached a post-thaw motility of (65.83±3.76)%, which was significantly higher than other samples (P<0.0001). Using cryopreservation solution B (containing 30 g/L trehalose, 10%DMSO and saline), the post-thaw motility was (67.92±3.96)%, which is signifi-cantly higher than those using the other two cryopreservation solutions. Samples frozen with optimized program (cryopreservation solution B, height of 7 cm) could achieve good fertilization rate (74.55±4.31)%, which was sig-nificantly lower than that of the corresponding fresh sperm (87.42±4.63)%, P<0.05). After two week’s preservation at 4℃, the red spotted grouper sperm motility reduced to 0.% 对赤点石斑鱼(Epinephelus akaara)精子的激活特点、超低温冷冻方案筛选、短期保存、受精实验等方面进行了研究。结果表明：水温25℃、比重为1.018的海水对赤点石斑精子具有最佳的激活效果,活力为(82.50±4.18)%；在进行超低温冷冻时,选择距离液氮面7 cm的高度进行降温,获得冻后活力为(65.83±3.76)%,显著高于1、3、15 cm 的处理组(P<0.0001),利用冻存液 B(含30 g/L 海藻糖、10%DMSO 的生理盐水)冻存赤点石斑精子,冻精活力为(67.92±3.96)%,显著高于另外两种冻存液(P<0.05)；通过优化的方案(冻存液B,7 cm高度)冻存得到精子可获得较好的受精效果,受精率为(74.55±4.31)%,而相应的鲜精受精率可达(87.42±4.63)%,二者间的差异具有显著性(P=0.017)；短期保存的结果表明,赤点石斑鱼精子在4℃环境中保存约两周后活力降低为0。
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