日本仙菜来源内生真菌Aspergillus versicolor EN-298化学成分研究

摘要

从采自烟台养马岛的日本仙菜(Ceramium japonicum)中分离得到的一株内生真菌Aspergillus versicolor EN-298,对其次级代谢产物进行了系统研究,利用反相柱层析、正相柱层析、葡聚糖凝胶Sephadex LH-20以及制备薄层层析(pTLC)等分离手段获得10个单体化合物。通过一维、二维核磁共振技术(1D、2D NMR)等鉴定了所有化合物的结构。并对分离得到的化合物进行了抑菌活性测试,其中化合物10对迟缓爱德华氏菌(Edwardsiella tarda)表现出一定的抑制作用。%Ten compounds were isolated from the secondary metabolites ofAspergillus versicolor EN-298, an endophytic fungus that was isolated from the marine red algaCeramium japonicum. The structures of the isolated compounds were elucidated by means of spectroscopic methods including UV and NMR (1D and 2D) as well as by comparison with authentic standards and literature reports. All compounds were evaluated for antimicrobial activity, and the compound10 showed moderate activity against aqua-bacterialEdwardsiella tarda with an MIC value of 16μg/mL.