红外三级鉴定法快速鉴别龙虾的4个品种

摘要

为鉴别不同龙虾(Panulirus),作者选用一维红外光谱(FTIR)、二阶导数谱(SDIR)和二维相关红外光谱(2DIR)来逐步提高谱图的分辨率, 使得4个品种的差异性逐渐明显.结果表明: 在一维谱图上, 波士顿龙虾(Homarus americanus)没有酰胺Ⅱ带的特征峰;日本龙虾(Panulirus japonicus)在1333 cm–1处有尖峰.在二阶导数谱上, 日本龙虾在1333 cm–1处尖峰更明显, 且在1535cm–1处有吸收峰, 与其他品种的差异表现得更明显.波纹龙虾(Panulirus homarus)在 1567cm–1处有尖峰,锦绣龙虾(Panulirus ornatus)在1567 cm–1处的峰弱而不明显,故可以区分出波纹龙虾和锦绣龙虾.在二维相关红外谱图上,进一步佐证了4种龙虾所含的氨基酸、蛋白质在种类和含量上有所差异.所以红外三级鉴定法可以对龙虾进行快速、有效的鉴别.%This study was performed to accurately identify the different species of lobsters using the IR analysis. We used the one-dimensional infrared spectra obtained (FTIR), the second derivative spectra (SDIR), and the two-dimensional correlation infrared spectroscopy (2DIR) to increase the spectral resolution gradually, which cleared the differences between four species of lobsters.The results demonstrated that Homarus americanus showed no amide peaks Π band on the one-dimensional spectrum,whereas Panulirus japonicus showed a peak at 1 333 cm–1. On the second derivative spectra,P.japonicus showed a more apparent peak at 1 333 cm?1and an obvious peak at 1 567 cm–1,which was obviously different from the other three samples;P.homarus showed a sharp peak at 1 567 cm–1, whereas P.ornatus showed no obvious peak at 1 567 cm?1,which could distinguish the two species.Further,on the two-dimensional correlation infrared spectra, the four samples showed different types of amino acids and proteins, and the content of amino acids and proteins was also different. We found that the IR analysis method could identify the four species of lobsters rapidly and effectively.