靶向抑制miRNA-21提高白血病K562细胞对阿糖胞苷的敏感性

摘要

Our goal is to investigate the influences of anti-miRNA-21 oligonucleotide (AMO-miR-21) on Ara-C chemosensitivity in human leukemic K562 cells,which were incubated with different concentration of Ara-C alone or in combination with AMO-miR-21.The growth-inhibitory potencies were measured by MTT assay.The inhibition rate and IC50 was calculated.The cell apoptosis was assessed by flow cytometry.The expression of miRNA-21 and its potential target gene PdcdA was detected by real-time PCR and Western blot in K562 cells.Transfection of K562 cells with AMO-miR-21 enhanced the chemosensitivity of Ara-C by up to2.3-fold and promoted Ara-C-induced cell apoptosis.Suppressing the cellular levels of miRNA-21 significantly upregulated the expression of PdcdA.The results support a substantial role for AMO-miR-21 that enhance Ara-C sensitivity in K562 cells.The mechanism appears to be related to anti-miRNA-21-induced apoptosis,suggesting a novel potential approach to treatment of leukemia.%为研究以miRNA-21为靶标的反义核酸(AMO-miR-21)提高白血病K562细胞对阿糖胞苷(Ara-C)的敏感性及可能的作用机制,在LipofectamineTM 2000介导下,将化学合成的AMO-miR-21转染K562细胞,四甲基偶氮唑蓝(MTT)法检测单独使用AMO-miR-21、Ara-C以及两者联合使用对细胞增殖抑制作用,并计算抑制率和IC50；流式细胞仪检测细胞凋亡；实时定量PCR(Real-time PCR)检测miRNA-21及其候选靶基因Pdcd4 mRNA的表达水平；免疫印迹(Western blot)检测Pdcd4蛋白的表达水平.结果显示Ara-C与AMO-miR-21联合使用后,IC50从1.95 μmol/L降低到0.84 μmol/L,敏感性提高到单用Am-C的2.3倍.AMO-miR-21联合Ara-C组细胞凋亡明显增加(P＜0.05).AMO-miR-21显著抑制K562细胞中miRNA-21的表达水平(P＜0.05),同时Pdcd4的表达明显增加(P＜0.05).提示AMO-miR-21可以提高K562细胞对Ara-C的敏感性,其作用机制与靶向抑制miRNA-21,进而诱导细胞凋亡有关.