△Np63基因在膀胱移行细胞癌尿液和血液中表达的研究

摘要

To explore the expression and clinical significance of delta Np63 mRNA and protein in urine and blood of the transitional cell carcinoma of the bladder (TCCB).The reverse transcriptase-polymerase chain reaction (RT-PCR) assay was conducted to detect expression of delta Np63 mRNA in urine of 53 cases of TCCB,40 cases of normal urine and 30 cases of peer age group urine,the expression of delta Np63 mRNA in blood of 47 cases of TCCB,27 cases of normal blood and 30 cases of peer age group.Pathological types and clinical stages of delta Np63 mRNA expression was also analyzed.The expression rate of delta Np63 mRNAin urine of TCCB was 71.7% (38/53) and in blood was 76.6% (36/47),respectively.The differences expression rate of delta Np63 mRNA in different pathological types and clinical stages of TCCB were not significant.There were high expression of delta Np63 mRNA in TCCB,that means delta Np63 maybe associated with tumorigenesis in TCCB,but may not be associated with pathological types and clinical stages of TCCB.Additional studies will be required to explore the function of delta Np63 in the progression of TCCB.%探讨△Np63 mRNA在膀胱移行细胞癌(transitional cell carcinoma of the bladder,TCCB)尿液和血液中的表达及意义.采用逆转录聚合酶链反应(RT-PCR)方法检测53例TCCB患者尿液,40例正常对照组尿液,30例患者同龄对照组;47例CCB患者血液,27例正常对照组血液中△Np63 mRNA的表达,30例患者同龄对照组.并分析ANp63 mRNA表达与TCCB临床分期、病理分级等的关系.结果发现TCCB患者尿液中△Np63 mRNA表达的阳性率为71.7％ (38/53),TCCB患者血液中△Np63 mRNA表达的阳性率为76.6％ (36/47),TCCB组尿液和血液中△Np63 mRNA表达的阳性率明显高于正常组,差异有显著性（P＜0.01),尿液和血液中△Np63 mRNA 表达的阳性率比较无统计学意义（P＞0.05）.不同临床分期和病理分级尿液与血液中△Np63 mRNA表达的阳性率,各组间差异无显著性(P＞O.05).TCCB组尿液和血液中△Np63 mRNA表达水平均明显高于正常组,提示△Np63与TCCB的发生密切相关,可能为临床早期诊断TCCB提供一个新的指标；△Np63 mRNA表达水平和TCCB的临床分期、病理分级没有明显相关性,推测△Np63起作用可能主要在TCCB发生阶段.