四氯化碳联合二乙基亚硝胺对小鼠肾脏毒性作用的研究

摘要

The toxic effect of carbon tetrachloride (CCl4) combining with diethylnitrosamine (DEN) on kidney was studied.Kunming mice (Mus musculus domesticus) were randomly divided into two groups.The experimental group was injected intraperitoneally with 15％ CCl4 in peanut oil twice a week for eight weeks,with free access to water containing 0.07％ DEN,and the control group was injected with same dose of peanut oil with free access to purified water.The diet and weight changes of mice were observed.In both experimental and control groups,four mice were killed at weeks 3,6 and 8,respectively.The kidney tissue was fixed,embedded and sectioned conventionally,and then stained by HE method.The structure and the shape changes of kidney tubules and collecting tubules were observed.The results showed that average mouse weight was significantly higher in the control group than in the experimental group.The structures of kidney in control group were distinct,arranged orderly,while the kidney tissues of experimental group had remarkable degeneration and necrosis and were more and more disorderly.The kidney tubules and collecting tubules showed a dramatic change,with the lumen narrowing down.These results proved that CCl4 has a damage effect on kidney and the damage is more and more serious with the extension of treatment time.%为了研究四氯化碳(carbon tetrachloride,CCl4)联合二乙基亚硝胺(diethylnitrosamine,DEN)对小鼠肾脏组织的毒性损伤作用,将30只雄性昆明鼠随机分为对照组和实验组,其中实验组小鼠每周2次腹腔注射15％CCl4花生油溶液,并且自由饮用0.07％ DEN溶液;而对照组小鼠注射等量的纯花生油,自由饮用纯净水,观察并记录各组小鼠的饮水、进食情况和体重变化,以及生活状态的改变.在实验过程中,分别于第3、6、8周解剖两组小鼠各4只,取其肾脏组织样本进行病理方面的组织切片观察.体重统计结果显示,与对照组相比,实验组小鼠体重明显下降.同时,实验组小鼠的肾脏系数明显高于对照组,并且具有显著差异(P＜0.05).组织切片观察发现,对照组小鼠肾脏组织结构比较清晰,排列较规则,而实验组小鼠肾脏组织呈现出显著的变性和坏死;且随着处理时间的延长,肾脏组织变得散乱无规则,细胞凝集现象越来越明显,贤小管与集合小管管腔变窄,颗粒样变性.上述结果表明CCl4联合DEN对小鼠肾脏具有损伤效应,处理时间越长,损伤作用越严重.