摘要:
Objective To study the effect of allicin on oxidative stress and inflammatory response of rats with chronic renal failure (CRF). Methods Totally 100 rats were randomly divided into normal control group, model control group and allicin 5, 10 and 20 mg/ (kg·d) groups. The CRF rat model was established by intragastric administration of adenine solution 250 mg/ (kg·d) for 21 d, and then the rats were given intraperitoneal injection of allicin 5, 10 and 20 mg/ (kg·d) once daily for 28 d. The 24 h urine output, 24 h urine protein and the kidney indexes were detected; the levels of serum urea nitrogen (BUN), serum creatinine (SCr) and uric acid (UA) were measured; the kidney index was calculated; the pathological changes of renal tissue was observed. The activities of antioxidant enzymes and malondialdehyde (MDA) in renal tissue and the levels of T-AOC and AOPPs in serum were measured. The levels of CRP, IL-1, IL-6, TNF-α, sICAM-1 and VCAM-1 in serum were detected by ELISA method. Results Compared with model group, the 24 h urine output, 24 h urinary protein, the kidney index and the contents of BUN, SCr and UA in serum in allicin 10 and 20 mg/ (kg·d) groups were significantly reduced (P<0. 05 or P<0. 01); allicin could significantly improve the renal tissue lesions of CRF rats. The activities of antioxidant enzymes (SOD) were significantly increased in the renal tissues, while the levels of MDA and serum AOPPs were significantly decreased (P<0. 05 or P <0. 01); the activities of catalase (CAT) in renal tissue and the level of T-AOC in serum of 20 mg/ (kg·d) group were significantly increased (P<0. 05). The content of CRP, TNF-α, sICAM-1 and VCAM-1 in serum of allicin 10 and 20 mg/ (kg·d) groups were significantly decreased (P<0. 05 or P<0. 01); the contents of IL-1 and IL-6 in allicin 20 mg/ (kg·d) group were significantly decreased (P<0. 05 or P<0. 01). Conclusion Allicin perhaps has protective effects on CRF rats by inhibiting oxidative stress injury and inflammatory response in renal tissue.%目的 研究大蒜素对慢性肾衰 (Chronic renal failure, CRF) 大鼠肾脏组织氧化应激损伤和炎症反应的影响.方法100 只实验用大鼠随机分为正常对照组, 模型对照组, 大蒜素5、10、20 mg/ (kg·d) 组, 采用腺嘌呤溶液250 mg/ (kg·d) 灌胃21 d 的方法建立CRF 大鼠模型, 大蒜素5、10、20 mg/ (kg·d) 组腹腔注射给药治疗, 疗程为28 d.测定24 h 尿量、24 h 尿蛋白量及血清肾功能指标 (BUN、SCr、UA 含量), 计算肾脏指数; HE 染色观察肾脏组织病理变化;化学比色法测定肾脏组织匀浆中抗氧化酶活性和丙二醛 (MDA) 含量, 血清中总抗氧化能力 (T-AOC) 和晚期氧化蛋白产物 (AOPPs) 水平; ELISA 法测定血清炎症细胞因子C 反应蛋白 (CRP) 、白细胞介素 (IL) -1、IL-6、肿瘤坏死因子-α (TNF-α) 、可溶性细胞间黏附分子-1 (sICAM-1) 、血管间黏附分子-1 (VCAM-1) 的水平.结果 与模型对照组比较, 大蒜素10、20 mg/ (kg·d) 组24 h 尿量减少、24 h 尿蛋白量降低 (P<0. 05 或P <0. 01), 肾脏指数降低 (P<0. 01), 血清BUN、SCr、UA 含量降低 (P<0. 05 或P<0. 01).大蒜素治疗明显改善 CRF 大鼠肾脏组织病变.大蒜素10、20 mg/ (kg·d) 组肾脏组织超氧化物歧化酶 (SOD) 活性升高, 但MDA、血清 AOPPs 水平降低 (P<0. 05 或P<0. 01); 20 mg/ (kg·d) 组肾脏组织过氧化氢酶 (CAT) 活性和血清T-AOC 水平均升高 (P<0. 05).大蒜素10、20 mg/ (kg·d) 组血清CRP、TNF-α、sICAM-1、VCAM-1 含量降低 (P<0. 05 或P <0. 01); 20 mg/ (kg·d) 组IL-1、IL-6 含量显著降低 (P<0. 05 或P<0. 01).结论 大蒜素可能通过抑制肾脏组织氧化应激损伤和炎症反应而对CRF 大鼠起到一定的保护作用.