高脂高糖食物诱导中缅树鼩肥胖的研究

摘要

为了研究高糖高脂食物诱导中缅树鼬肥胖的模型,在高脂高糖食物诱导条件下进行了中缅树鼩的血液指标测定、脂肪组织流式细胞分析和正电子发射计算机断层显像(positron emission tomography/computed tomography,PET/CT)扫描成像研究.结果表明:在28 d时中缅树鼩白色脂肪组织(white adipose tissue, WAT)质量是对照组的3.7倍,49 d时WAT质量是对照组的6.4倍;49 d时实验组树鼩的甘油三酯(triglyceride, TG)和高密度脂蛋白(high density lipoprotein, HDL)含量与对照组树鼩差异显著,血糖(blood glucose)水平、总胆固醇(total cholesterol, CHOL)和血小板(blood platelet, PLT)含量与对照组树鼩差异极显著;此外,食物诱导28 d组中缅树鼩腹部WAT和褐色脂肪组织(brown adipose tissue, BAT)的18氟-氟化脱氧葡萄糖(18-fluoro-fluo-rodeoxyglucose, 18F-FDG)吸收60 min后,平均辐射值(standardized uptake value, SUV)显著高于对照组,49 d时有所下降.流式细胞分析结果表明,28 d时WAT和BAT中R2群标记阳性显著增加,而49 d后有所降低.以上结果表明:食物诱导可以使中缅树鼩产生肥胖并显著增加中缅树鼩WAT的质量,中缅树鼩在28 d时会出现肥胖抵抗的现象,但是随着时间的延长,该抵抗现象逐渐减少.%In order to investigate the high-fat and high-sugar diet-induced obesity model in Tupaia belangeri, blood index measurement, flow cytometry analysis and positron emission tomography/computed tomography (PET/CT) scanning on adipose tissue of T. belangeri were performed under the condition of high-fat and high-sugar food in the present study. The results showed that the mass of white adipose tissue (WAT) was 3.7 times heavier than that of the control group on day 28,and was 6.4 times heavier than that of the control group on day 49. The triglyceride (TG) and high density lipoprotein (HDL) levels were significantly higher than those of the control group on day 49,and the blood glucose levels, total cholesterol (CHOL) and blood platelet(PLT) levels showed extremely significant differences between experimental group and control group on day 49. Sixty minutes after absorption of 18-fluoro-fluorodeoxyglucose (18F-FDG) in WAT and brown adipose tissue(BAT), the standardized uptake value (SUV) was significantly higher than that of the control group on day 28. But on day 49,the SUV declined. Flow cytometry analysis showed that positive markers of R2 group in WAT and BAT increased significantly on day 28, and decreased on day 49. All of the results showed that high-fat and high-sugar food could induce obesity and increase the mass of WAT significantly in T. belan-geri. The obesity resistance phenomenon may appear on day 28, but with the extension of acclimation time, it will gradually reduce.