Sub-chronic toxicity of the aqueous wood-ash extract of Parkia biglobosa in Mus musculus

摘要

Considering the possible health hazards associated with consumption of aqueous wood-ash extracts,this study investigated the sub-chronic toxicity of aqueous wood-ash extract of Parkia biglobosa on Mus musculus.Parkia biglobosa(5 kg)were collected and aqueous extracts of wood-ash were prepared by percolation method.Doses of 0(control),5,50 and 100 mg/kg,once a day,were administered to mice(n=10/treatment)90 days.Blood was collected by retro-orbital phlebotomy and assayed for serum creatinine,aspartate aminotransferase and alanine aminotransferase,and the kidney and liver were excised for histopathology using standard methods.Data were analysed using descriptive statistics and ANOVA atα0.05.Serum creatinine(mg/dL),aspartate aminotransferase(UI/L)and ALT(UI/L)increased with dosage and peaked at 100 mg/kg:2.1±0.2,128.8±8.0 and 58.0±2.9 compared to the control:0.8±0.1,89.8±5.1 and 28.5±1.4.Tubular degeneration and necrosis in the kidney,hepatocyte atrophy and dissociation of cords in the liver were observed at the 100 mg/kg dose level,while control organs had normal tissue architecture.Aqueous wood-ash extracts of P.biglobosa showed sub-chronic toxicity in Mus musculus.