矮牵牛的离体再生与快速繁殖

摘要

矮牵牛带芽嫩茎、嫩叶作为外植体,经培养都能再生完整植株.诱导分化阶段,带芽嫩茎以腋芽发育为主,也有不定芽发育,最适培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L或MS+6-BA1.0 mg/L+NAA0.5 mg/L.叶片以不定芽发育为主,在MS+6-BA0.5 mg/L+NAA0.5 mg/L及MS+6-BA1.0 mg/L+NAA0.1 mg/L培养基上,丛生芽分化效果最好.继代增殖阶段,切割新梢接种于MS培养基或切割丛生芽接种于MS+6-BA0.5 mg/L培养基,均可获4～5倍的增殖.生根培养阶段,MS+IBA0.01～0.05 mg/L培养基的生根效果好,移栽成活率可达95.6%.