黄瓜叶片蛋白质双向电泳条件优化

摘要

This paper establishes cucumber leaf protein two-dimensional electrophoresis conditions optimization , which to extract leaf total soluble protein using TCA / acetone precipitation method, with 600 μg sample to electrophoresis, staining with Coomassie brilliant blue G-350. It is obtained a stable of cucumber leaf protein two-dimensional electrophoresis system.%建立了黄瓜叶片总蛋白双向电泳技术优化体系,即可溶性蛋白提取方法为TCA/丙酮沉淀法,上样量为600μg,染色方法为考马斯亮蓝G-350.从而获得一个比较稳定的黄瓜叶片蛋白质双向电泳体系.