慢性髓细胞性白血病病人骨髓单个核细胞中差异表达基因的筛选

摘要

Objective: To screen differential expressed genes in marrow mononuclearcell of chronic myelogenous leukemia (CML) patient in order to develop the mechanism of CML.Methods: RNA were extracted in marrow mononuclearcell of both normal person and CML patient marked with digoxin.Differential expressed genes were analyzed by ABI 1700 bioarray and sorted by Jubilant physiology/disease categories.Results: A total of 6706 genes had significant differential expressed 43 genes relating to CML which have 13 genes up-regulated relating to signal transduction, protein metabolism and modification, nucleoside, nucleotide and nucleic acid metabolism, mRNA transcription, et al; while 30 genes down-regulated relating to nucleoside, nucleotide and nucleic acid metabolism, protein metabolism and modification, signal transduction, developmental processes, cell cycle, et al.Conclusion: 43 genes relating to CML have significant differential expressed, and play an important role in the process of CML malignant transformation.%目的:筛选慢性髓细胞性白血病(CML)病人骨髓单个核细胞与正常人的差异表达基因,探讨CML的发病机制.方法:提取正常人和CML病人单个核细胞的RNA,逆转录成cDNA并用地高辛标记,应用全基因组表达谱基因芯片对差异表达基因进行研究,采用Jubilant病理/疾病分类法对CML相关差异表达基因进行分析.结果:共筛选出CML相关差异表达基因43个,其中13个基因表达上调,30个基因表达下调,上调基因较多地参与信号转导、蛋白代谢、修饰、核酸代谢、mRNA转录调控等生物学过程;下调的基因主要涉及核酸代谢、信号转导、细胞及个体发育、细胞周期、免疫及防疫等方面.结论:CML病人与正常人的单个核细胞中有43个基因具有显著的表达差异,筛选获得的差异靶基因在CML恶性转化过程中发挥了重要作用,这为药物靶基因的筛选提供了理论基础.