2种太岁样品中微生物的分离和鉴定

摘要

Objective: Taisui is an organism with undefined classification existed in nature. The object of this study was to isolate and molecular identity strains in two Taisui samples obtained from different source by molecu- lar method. Methods: After washing with 75% ethanol for about 10 s, the Taisui samples were inoculated into malt wort medium to obtain the cultured strains. Genomic DNA of Taisui samples were isolated and used as the template to amplify 16S rDNA, 18S rDNA and ITS by PCR. The PCR fragments were then ligated into pMD18T and sequenced respectively. Strain identification was performed by sequence alignment against NCBI GenBank. Re- sults: Candida and Rhodotorula mucilaginosa were found in No.1 sample while Agrobacterium tumefaciens and R. mucilaginosa were found in No.2 sample. Conclusion: Rhodotorula mucilaginosa was found in these two Taisui samples from different source.%目的：太岁是自然界中一种组成及分类尚不明确的生物体。采用分子生物学方法对2种不同来源地的太岁样品进行菌种分离培养和分子鉴定。方法：太岁表面经75%乙醇消毒处理后，用麦芽汁培养基分离可培养菌株，提取太岁及分离菌株基因组DNA，以之为模板，用16S rDNA、18S rDNA和ITS通用引物进行PCR扩增，扩增片段连入pMD18T载体并测序，通过序列比对对太岁菌种组成进行初步鉴定。结果：从太岁1号样品中分离到假丝酵母（Can-dida）和粘质红酵母（Rhodotorula mucilaginosa），从太岁2号样品中分离到根癌农杆菌（Agrobacterium tumefaciens）和粘质红酵母。结论：2种来源不同的太岁样品中均存在可培养的粘质红酵母。