植物转录因子最新研究方法

摘要

Transcription factors regulate vast downstream genes and play an important role in regulation of devel⁃opment, metabolism and response to environment in plants. In this review, we analyzed the recent research prog⁃ress in plant transcription factors and introduced strategies and current developed methodology. The main strategies including bioinformatic analysis, transient transfection, phenotypic mutation and regulatory network were discussed. Our review may provide some useful instruction for the future research of transcription factor in prediction, func⁃tional analysis and identification of targets.%　　转录因子可以调控众多下游基因的表达，在植物的生长发育、代谢及对外界环境的反应中起着重要作用。我们结合近年来植物转录因子的研究进展，归纳分析了高等植物转录因子研究的主要策略和最新的技术方法，并从生物信息学分析、瞬间转化技术的应用、突变体表型分析及调控网络等几个方面进行了全面阐述，为植物转录因子的预测、功能鉴定及靶基因分析等相关研究提供理论和方法的参考。