表皮葡萄球菌在慢性鼻-鼻窦炎患者中生物膜的表达

摘要

目的 研究表皮葡萄球菌生物膜在慢性鼻-鼻窦炎(CRS)患者鼻黏膜中的表达情况.方法 将临床手术中获取的180例慢性鼻-鼻窦炎患者内镜手术黏膜样本,根据细菌培养结果将其分为4组,分别 为表皮葡萄球菌生长组79例,其他葡萄球菌生长组33例,阴性杆菌生长组27例,细菌培养阴性组41例,所有样本均行标准的扫描电子显微镜方法检测.结果 表皮葡萄球菌生长组79例全部观察到生物膜的存在,表达率为100%(79/79),其他葡萄球菌生长组为75.8%(25/33),阴性杆菌生长组为77.8%(21/27),细菌培养阴性组为41.5%(17/41).后3组分别与表皮葡萄球菌组比较,P值均＜0.01.结论 表皮葡萄球菌在CRS患者生物膜形成中起重要作用.%Objective To investigate the expression of Staplzylococcus epidermidis biofilm in the nasal mucosa of patients with chronic thinosinusitis ( CRS ).Methods 180 samples were collected during endoscopic sinus surgery from patients with CRS.According to the results of bacterial culture, they were classified into 4 groups [ Staphylococcus epidermidis growth group ( 79 cases ), other Staphylococcus aureus growth group ( 33 cases ), negative bacillus growth group ( 27 cases ) and hacterial culture-negative group ( 41 cases ) ].All samples were subjected to scanning electron microscopy.Results The scanning electron microscopy analysis showed bacterial biofilms in all cases of the Staphylococcus epidermidis growth group, and the expressions for the 4 groups were l00％ ( 79/79 ), 75.8％ ( 25/33 ),77.8％ ( 21/27 ) and 41.5％ ( 17/41 ), respectively.The results were compared hetween Staphylococcus epidermidis growth group and other 3 groups ( P ＜ 0.01 ).Conclusions Staphylococcus epidermidis is important for the formation of biofilms in the patients with CRS.