妊娠期女性血细胞主要参数生物参考区间建立的初步探讨

摘要

目的：建立本实验室妊娠期女性血细胞主要参数的生物参考区间。方法选取331例正常足月分娩的孕妇，追溯其孕期检查记录，用 SAS 8．1软件对孕期间进行的2次血细胞常规检测结果进行统计分析，获得参考区间。结果比较妊娠早期和妊娠晚期的各项血常规检验结果，差异具有统计学意义（P 均＜0．05）。参考区间分析显示血小板（PLT）、单核细胞百分比（M ％）和嗜碱性粒细胞百分比（B ％）检验结果均在现行参考区间内，故继续沿用原有参考区间；血细胞比容（HCT）和嗜酸性粒细胞百分比（E ％）检验结果，经验证可合并参考区间；而红细胞计数（RBC）、血红蛋白（Hb）、白细胞计数（WBC）、中性粒细胞百分比（N ％）及淋巴细胞百分比（L％）需分组建立不同的参考区间。结论新建的生物参考区间更符合孕期的生理变化，能更好的指导临床诊断和治疗。%Objective To establish the biological reference intervals about the main parameters of blood cells among pregnant women in our laboratory.Methods A total of 331 pregnant women who had normal and term delivery were enrolled.According to their medical records,their 2 reports of pregnancy routine blood cell tests were obtained. The results were analyzed statistically,and the biological reference intervals were calculated by SAS 8.1 software. Results There was statistical significance in all parameters of pregnancy routine blood cell tests between the first and third trimesters (P <0.05).The results of biological reference intervals showed that the 2 sets of data about platelet (PLT),monocyte percentage (M%)and basophilic granulocyte percentage (B %)were all within current biological reference intervals,then the current biological reference intervals could be used for pregnancy.The 2 sets of data about hematocrit (HCT)and eosinophile granulocyte percentage (E %)could be combined to new biological reference intervals.The biological reference intervals for red blood cell count (RBC),hemoglobin (Hb),white blood cell count (WBC),neutrophile granulocyte percentage (N %) and lymphocyte percentage (L %) should be established according to different trimesters.Conclusions The newly established biological reference intervals are appropriate to reflect the physiological changes during pregnancy,and can guide the clinical diagnosis and treatment effectively.