高原地区慢性阻塞性肺疾病患者IL-8、IL-17和MMP-9水平研究

摘要

Objective To investigate interleukin 8 (IL-8 ),interleukin 1 7 (IL-1 7 )and matrix metalloproteinase (MMP-9)levels among patients with chronic obstructive pulmonary disease (COPD)in high altitude area.Methods By enzyme-linked immunosorbent assay (ELISA ),among COPD patients in acute exacerbation and remission and healthy subjects,the serum IL-8,IL-1 7 and MMP-9 levels were determined,analyzed and compared statistically. Results The IL-8,IL-1 7 and MMP-9 levels in COPD patients in acute exacerbation [(9.1 6 ±2.91 ),(1 2.48 ± 2.1 2)and (4.89 ±0.99)ng/L]and remission [(6.53 ±1 .96),(5.76 ±1 .98)and (3.24 ±0.97)ng/L]were higher than those in healthy controls,and the difference was statistically significant (P <0.05 ).Those in acute exacerbation were significantly higher than those in remission.Conclusions The determinations of IL-8,IL-1 7 and MMP-9 levels can be used as the theory indices of COPD diagnosis in high altitude area.%目的：探讨高原地区慢性阻塞性肺疾病（COPD）患者血液中白细胞介素-8（IL-8）、白细胞介素-17（IL-17）和基质金属蛋白酶（MMP-9）水平变化。方法采用酶联免疫吸附试验（ELISA）检测COPD急性加重期及缓解期患者、健康人血清IL-8、IL-17和MMP-9水平并进行统计学分析比较。结果 COPD急性加重期、缓解期患者血清IL-8、IL-17和MMP-9水平分别为[（9．16±2．91）、（12．48±2．12）、（4．89±0．99）ng/L]、[（6．53±1．96）、（5．76±1．98）、（3．24±0．97）ng/L]均高于健康对照组，差异有统计学意义（P＜0．05）；急性加重期血清IL-8、IL-17和MMP-9检测水平也明显高于缓解期。结论 IL-8、IL-17和MMP-9水平检测可作为高原地区COPD诊断和观察疗效的理论指标。