VIPR2拷贝数变异检测方法的建立及应用

摘要

Objective To establish a method detecting VIPR2 copy number variation ,and to apply it in the a-nalysis on the relationship between VIPR2 copy number variation and schizophrenia .Methods A total of 310 patients with schizophrenia from Nov .2010 to Aug .2012 in Mental Health Center in Wuxi City Affiliated to Nanjing Medical University were selected into schizophrenia group ,and 300 healthy people were recruited into control group .Polymer-ase chain reaction(PCR)method were used to analyze the VIPR2 copy number .Results The positive rate of VIPR2 copy number variation in the schizophrenia group and control group were 0 .97% (3/310)and 0 .00% respectively ,and there was no significant difference between the two groups (χ2 = 1 .275 ,P= 0 .259) .Conclusion PCR could be an ef-fective method to confirm VIPR2 copy number variation .%目的：建立 VIPR2基因拷贝数变异检测方法，并应用于 VIPR2拷贝数变异与精神分裂症的相关性研究中。方法将2010年11月至2012年8月南京医科大学附属无锡市精神卫生中心收治的310例精神分裂症住院患者纳入精神分裂症组，同期选择300例健康志愿者纳入健康对照组。采用多重竞争性实时荧光定量聚合酶链反应法，对610例受试者进行 VIPR2微重复分析。结果精神分裂症组中 VIPR2拷贝数变异检测阳性率为0．97％（3/310），健康对照组 VIPR2拷贝数变异检测阳性率为0．00％（0/300），两组比较差异无统计学意义（χ2＝1．275，P＝0．259）。结论多重竞争性聚合酶链反应法能对多位点拷贝数变异进行快速分型，是验证拷贝数变异的有效方法。