丙型肝炎病毒抗体联合核酸定量检测对丙型肝炎患者的早期诊断价值研究

摘要

Objective To explore the value of hepatitis C virus antibody (HCV Ab) and hepatitis C virus nu-cleic acid (HCV RNA ) for the diagnosis of hepatitis C .Methods Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect HCV RNA ,enzyme-linked immunosorbent assay (ELISA) and colloidal gold labeled method was used to detect HCV Ab ,and the rate method was used to detect alanine aminotransferase (ALT ) in 116 patients with hepatitis C .Results The positive ratios of HCV Ab detected by ELISA ,HCV RNA , HCV Ab detected by colloidal gold labeled method and ALT were respectively 83 .6% ,73 .3% ,66 .4% and 59 .5% . Positive rated of joint determination of HCV Ab and HCV RNA was 100 .0% .Conclusion Combined detection of HCV Ab and HCV RNA could be conducive to the diagnosis of hepatitis C .%目的：探讨在丙型肝炎患者早期诊断中丙型肝炎病毒抗体联合丙型肝炎病毒核酸（HCV RNA）检测的临床价值。方法对116例鼓楼医院住院及门诊丙型肝炎患者采用实时荧光定量聚合酶链反应（FQ-PCR）测定HCV RNA、酶联免疫吸附试验（ELISA）和金标法测 HCV抗体、速率法测丙氨酸氨基转移酶（ALT ）。结果116例丙型肝炎患者中，ELISA HCV抗体阳性97例（占83．6％），HCV RNA阳性85例（占73．3％），HCV 金标阳性77例（占66．4％），ALT阳性69例（占59．5％），HCV抗体和 HCV RNA联合检测总阳性率（指任一指标阳性即为阳性）为100．0％。结论 HCV抗体和HCV RNA联合检测扩大了丙型肝炎检测范围，降低了丙型肝炎的漏诊率，有利于丙型肝炎的早期诊断。