Cella Vision DM96自动成像系统在白细胞分类计数中的应用

摘要

Objective To evaluate the value of CellaVision DM 96 automated digital cell morphology and infor-matics system (DM96 system) for leucocyte differential count in peripheral blood smears .Methods A total of 112 samples treated with dipotassium ethylenediamine tetraacetic acid (EDTA-K2 ) and needed reclassification after detec-tion of LH750 hematology analyzer were collected .Leucocyte differential counts of each sample were performed with both DM96 system and microscopy .Data were analyzed with Microsoft Excel software .Results There was fine cor-relation of classification of unidentified cells between DM 96 system and manual detection with microscopy ,and the correlation coefficients of segmented neutrophils ,band neutrophils ,lymphocytes and eosinophils were 0 .98 ,0 .93 , 0 .97 and 0 .94 ,respectively .Conclusion DM96 system could supply correct and reliable leucocyte differential data as a novel automated instrument in the hematology laboratory and might be suitable for clinical application .%目的：探讨 CellaVision DM96自动成像系统对外周血涂片中白细胞分类计数的应用价值。方法采用2011年7月1日至8月31日沈阳军区总医院住院和门诊患者的乙二胺四乙酸二钾抗凝静脉血，在贝克曼库尔特L H750血液分析仪检测需复检的112例标本，同时用CellaVision DM 96系统和显微镜法进行复检，两组数据采用微软Office Excel软件进行统计学分析。结果 CellaVision DM 96系统与显微镜手工分类有着很好的相关性，其中中性分叶核粒细胞、中性杆状核粒细胞、淋巴细胞及嗜酸性粒细胞的相关系数分别达到0．98、0．93、0．97和0．94。结论 CellaVision DM96系统作为一种全自动血细胞形态鉴定仪器，能够给临床实验室提供准确、可靠的白细胞分类数据，适于临床推广应用。