首发精神分裂症患者外周血Reelin基因甲基化检测

摘要

目的：通过精神分裂症患者外周血中Reelin基因启动子区DNA甲基化状态的研究，探讨DNA甲基化与精神分裂症的关联。方法收集精神分裂症患者76例（患者组）和健康对照者60例（健康对照组），采用甲基化特异性PCR方法检测Reelin基因启动子区DNA甲基化状态。结果患者组甲基化阳性率为78．95％（60例），健康对照组甲基化阳性率为35％（21例），两组差异有统计学意义（χ2＝4．971，P＝0．026）；Logistic回归分析显示， Reelin基因甲基化与精神分裂症有关（OR＝13．084，95％ CI：5．079～26．308）。结论 Reelin基因启动子区DNA甲基化与精神分裂症有关，精神分裂症患者外周血中Reelin基因启动子区DNA呈超甲基化状态，Reelin基因DNA甲基化是精神分裂症的危险因素。%Objective To study the relationship between DNA methylation with schizophrenia by investigating the DNA methylation status of peripheral blood Reelin gene promoter in the patients with schizophrenia .Methods The Reelin gene promoter DNA methylation was measured in 76 patients with schizophrenia(patients group) and 60 healthy controls(healthy control group) by adopting methylation‐specific PCR .Results The methylation positive rate in the patients group was 78 .95% (60 cases) ,which in the healthy control group was 35% (21 cases) ,the differ‐ence was statistically significant(χ2 =4 .971 ,P=0 .026);the Logistic regression analysis showed that the Reelin gene methylation was correlated with schizophrenia(OR=13 .084 ,95% CI:5 .079-26 .308) .Conclusion The Reelin gene promoter DNA methylation is associated with schizophrenia ,and the peripheral blood Reelin gene promoter DNA methylation presents the supermethylation status in the patients with schizophrenia .Reelin gene DNA methylation is the risk factor for schizophrenia .