Objective To make the results of drug resistance monitoring to better reflect the current situation of drug resistance by analyzing the the drug resistance characteristics of Klebsiella pneumoniae separated from differ‐ent specimens sources .Methods Klebsiella pneumoniae was isolated from the clinical specimens from different parts . The bacterial identification and drug susceptibility test were performed by using VITEK 2 COMPACT automatic mi‐croorganism analyzer .Results There were 558 strains of Klebsiella pneumonia separated from different specimens ,in which 368 strains were separated from sputum and 190 strains were separated from other specimens .Of the 190 strains ,the first three were separated from urine (27 .37% ) ,blood (24 .21% ) and pus (12 .63% ) .116 strains (61 .05% ) were producing extendedβ‐lactamases(ESBLs) negative and 74 strains were producing ESBLs positive . Compared with ESBLs negative strains ,the resistance rates of ESBLs producing strains to ampicillin ,cefazolin ,ampi‐cillin/sulbactam ,trimethoprim/sulfamethoxazole ,gentamicin ,ciprofloxacin ,ceftriaxone ,levofloxacin ,tobramycin ,az‐treonam ,nitrofurantoin ,amikacin and cefotetan were significantly increased(P<0 .05) .52 strains of Klebsiella pneu‐monia were separated from the urine specimens during 2014 and their sensitivity to ertapenem ,imipenem ,ceftazidime and cefotetan was more than 90% .46 strains of Klebsiella pneumonia were separated from the blood specimens and the sensitivity to imipenem ,ceftazidime ,cefotetan and ertapenem was over 90% .368 strains of Klebsiella pneumonia were separated from the sputum specimens and their sensitivity toceftazidime ,imipenem ,ertapenem ,cefotetan and amikacin was over 90% .Compared with the drug resistance rate in strains isolated from the sputum specimens ,the resistance of Klebsiella pneumonia isolated from the blood and urine specimens to various antibacterial drugs had no statistical difference(P>0 .05) .Conclusion Differentiating the specimen type for conducting statistics by analysing the antibacterial drug sensitivity of Klebsiella pneumoniae strains can more accurately reflect the drug resistance sta‐tus of strains and better select antibacterial drugs in clinic .%目的：通过分析不同标本来源的肺炎克雷伯菌的耐药性特征，使耐药监测的结果能更好地反映医药耐药性的现状。方法分离临床送检不同部位标本的肺炎克雷伯菌，采用VITEK 2 COMPACT 全自动微生物分析仪进行细菌鉴定及药物敏感性试验。结果2014年临床送检的各类标本共检出肺炎克雷伯菌558株，其中痰标本检出368株，无菌标本共检出190株。无菌标本前3位标本来源分别为尿液52株（27．37％），血液46株（24．21％），脓液24株（12．63％）；其中产超广谱β内酰胺酶（ESBL ）阴性菌株116株（61．05％），ESBL阳性菌株74株（38．95％）。与ESBL阴性菌株比较，ESBL产酶株对氨苄西林、头孢唑林、氨苄西林／舒巴坦、复方磺胺甲噁唑、庆大霉素、环丙沙星、头孢曲松、左氧氟沙星、妥布霉素、氨曲南、呋喃妥因、阿米卡星、头孢替坦的耐药率明显升高（ P＜0．05）。2014年尿液标本检出肺炎克雷伯菌52株，尿液标本培养出的肺炎克雷伯菌对厄他培南、亚胺培南、头孢他啶和头孢替坦的敏感率均超过90％。血液标本共分离肺炎克雷伯菌46株，对头孢他啶、头孢替坦、亚胺培南和厄他培南的敏感率均超过90％。痰液标本共分离肺炎克雷伯菌368株，对头孢他啶、厄他培南、亚胺培南、头孢替坦和阿米卡星的敏感率均超过90％。与痰液标本分离菌株耐药率比较，血液和尿液分离肺炎克雷伯菌对各抗菌药物的耐药率差异无统计学意义（P＞0．05）。结论通过对菌株的抗菌药物敏感性区分标本类型进行统计，可以更准确反映菌株的耐药性状况，更好地指导临床抗菌药物的选用。
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