Dermokine-βmRNA在结肠癌组织中的水平及临床意义

摘要

Objective To investigate the level of dermokine‐β(DK‐β) mRNA in colon cancer tissue and its clin‐ical value .Methods The RT‐PCR method was used to detect DK‐β mRNA relative level .The DK‐βmRNA relative levels were compared among the patients with colon cancer ,colonic polyps and ulcerative colitis and normal control group .The DK‐βmRNA relative levels were compared among different clinical parameters in colon carcinoma .The statistical awalysis was performed .Results The expression of DK‐β mRNA in the patients with colon cancer was significantly higher than that in the patients with colon polyps ,ulcerative colitis and normal control group ,the differ‐ence was statistically significant(P< 0 .01) .But the DK‐β mRNA expression had no correlation with age ,gender , tumor location and size .The DK‐β mRNA expression in the low differentiation adenocarcinoma ,Dukes B stage and lymph node metastasis was more significant (P<0 .05) .Conclusion The DK‐βexpression in colon cancer is closely associated with mucosal epithelial cell damage .The DK‐β expression in the stage B is significantly increased .There‐fore ,the DK‐βlevel detection in colon tissue may be used as an index of early diagnosis of colon cancer ,which has a certain value for early diagnosis of colon cancer .%目的：研究Dermokine（DK）‐βmRNA在结肠癌组织中的水平和临床价值。方法采用RT‐PCR法检测DK‐βmRNA相对水平，对结肠癌患者、结肠息肉、溃疡性结肠炎患者以及健康对照组DK‐β mRNA相对水平进行比较，对结肠癌不同临床参数间DK‐βmRNA相对水平进行比较，并进行统计学分析。结果结肠癌患者DK‐βmRNA表达比结肠息肉、溃疡性结肠炎患者以及健康对照组明显升高，差异有统计学意义（P＜0．01）；但与患者年龄、性别、肿瘤的位置、大小无相关性，在低分化腺癌、Dukes分期B期、有淋巴结转移的患者中DK‐βmRNA表达更明显（P＜0．05）。结论结肠癌DK‐β表达与结肠癌患者黏膜上皮细胞受损密切相关，B期DK‐β表达呈显著升高，因此结肠组织DK‐β水平检测可作为结肠癌早期诊断的指标，对临床结肠癌早期诊断有一定价值。